Random positioning machine (RPM) as a ground based microgravity simulating platform was designed, fabricated and validated. Experiments using biological samples proved that the achieved microgravity levels (10-3g) significantly influenced many biological parameters and the results were also compared with data from spaceflight studies. The developed RPM can be effectively utilized for the ground based optimization of spaceflight experiments. (currently under patent filing: KG Sreejalekshmi and Srujana Juratagi)